DNA Cloning: Expression systems

DNA Cloning: Expression systems

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bacteriophage CE6, a lambda phage recombinant containing and expressing the T7 polymerase gene. ... 2.4 pQE vectors This series of vectors may be used as alternative histidine tagged vectors to allow affinity purification of fusion proteins on Ni2+ resin columns. ... rearrangements of plasmid DNA have been reported in DH5 derivative or XL- 1 blue strains (Pharmacia GST troubleshooting guide) .

Title:DNA Cloning: Expression systems
Author: David M. Glover, B. D. Hames
Publisher:Oxford University Press - 1995

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