Multidimensional Microscopy

Multidimensional Microscopy

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Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.System being so well adapted to detecting detail from slight variations in color hue. ... based on the 1976 CIE Uniform Scales Chromaticity Diagram, which indicated that these three wavelengths, when combined, would give ... In addition , an interesting and unexpected application for our color confocal microscope arose from investigations of immunogold-labelled microtubules in mitotic plant endosperm.

Title:Multidimensional Microscopy
Author: P.-C. Cheng, T.H. Lin, W.L. Wu, J.L. Wu
Publisher:Springer Science & Business Media - 2012-12-06

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