Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques

Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques

4.11 - 1251 ratings - Source

Decades of development of the polymerase chain reaction (PCR) have yielded a significant array of associated techniques that make it possible to rapidly detect low numbers of all known pathogenic microorganisms without the traditional, more taxing methods of cultivation and phenotypic characterization. Written by one of the most prolific and respected researchers in food safety, Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques describes the application of molecular techniques for the detection and discrimination of major infectious bacteria associated with foods. The book puts a particular focus on genes associated with pathogenicity used in PCR, including real-time PCR for specific detection of pathogenic bacteria and the inherent limitations of such methodology with certain pathogens. It also emphasizes methods for extracting microorganisms from complex food matrices and DNA purification techniques. The coverage begins with a highly comprehensive review of real time PCR, complete with theoretical and operational concepts. Each chapter deals with a specific organism and the techniques applied to that organism. The text includes references on the use of PCR primers and DNA probes, the DNA sequence of each being listed at the end of each chapter to create a complete compendium. This is not a qrecipe bookq, but rather a resource with sufficiently detailed information that allows readers to fully comprehend the methodology described and the significance of the results. Copiously illustrated with figures, tables, charts, and graphs, this is a detailed presentation of the major, contemporary studies involving the molecular detection, quantification, and subspecies differentiation of each organism. With objective assessments of the molecular techniques, their advantages, and limitations, the book allows investigators to readily identify the precise molecular technique and application most suitable for their research.Ng, L., Kingombe, C., Yan, W., Taylor, D., Hiratsuka, K., Malik, N., Garcia, M. 1997 . ... J. Clin. Microbiol. 38:3800a€“3810. Nogva, H., Bergh, A., Holck, A., Rudi, K. 2000. Application of the 5a€²-nuclease PCR assay in evaluation ... Proceedings of the Eighth International Workshop on Campylobacters, and Related Organisms, July 10a€“13, 1995, Winchester, UK. ... Heat injury and repair in Campylobacter jejuni.

Title:Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques
Author: Robert E. Levin
Publisher:CRC Press - 2009-10-26

You must register with us as either a Registered User before you can Download this Book. You'll be greeted by a simple sign-up page.

Once you have finished the sign-up process, you will be redirected to your download Book page.

How it works:
  • 1. Register a free 1 month Trial Account.
  • 2. Download as many books as you like (Personal use)
  • 3. Cancel the membership at any time if not satisfied.

Click button below to register and download Ebook
Privacy Policy | Contact | DMCA