Resin Microscopy and On-Section Immunocytochemistry

Resin Microscopy and On-Section Immunocytochemistry

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For the first time, fixation, resin embedding and immunocytochemistry, the subjects of innumerable publications, have been organised into a comprehensive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.14, diagram 2). The antisera must be from different animal species which do not crossreact. The secondary detection ... (A) could be blocked with unconjugated GAR to prevent it reacting with GAR10 as well as GAR5, and the spare ... The localisation of two primary reagents from the same species on the same section can be acheived sequentially by labelling one of the antisera with a hapten (Fig.

Title:Resin Microscopy and On-Section Immunocytochemistry
Author: Geoffrey R. Newman, Jan A. Hobot
Publisher:Springer Science & Business Media - 2012-12-06

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