The Involvement of Wnt Signaling in Osteoblastic Cell Behavior

The Involvement of Wnt Signaling in Osteoblastic Cell Behavior

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Wnt (wingless and int-related proteins) are a family of secreted cysteine-rich glyeoproteins, expressed in a variety of tissues in developing embryos and adult tissues, thought to be implicated in oncogenesis, embryonic development, and regeneration ofadult tissues. In our study, Wnt5a was found to be one of the predominant Wnts that are expressed during osteoblastic differentiation of hMSCs in vitro and during hMSC-directed ectopie osteogenesis in the severe combined immunodeficient (SCID) mouse host. RT-PCR further revealed that hWNT5A and its receptor Frizzled family member 5 (hFZD5) was up-regulated during osteoblastic differentiation. The function of Wntsa was further evaluated using knock-out model. Wnt5a -/- calvarial cells showed significantly slower cell proliferation when compared to Wnt5a-/- and wild type cells. Gene expression profiles of the Wnt5a-/- calvarial cells as compared to wild type cells were evaluated using microarray analysis. 255 genes exhibited at least 2-fold changes in expression. In addition, genes including Runx2, osterix, and alkaline phosphatase (ALP) were shown to be down-regulated in Wnt5a-/- cells. The results suggested that Wnt5a plays a role in ostcogcncsis.The cell numbers, excluding Trypan blue-positive cells, were determined by manual counting (triplicate wells) in a hematocytometer. Quantitative ... Results were analyzed using the Applied Biosystems 7500 Real-Time PCR System software.

Title:The Involvement of Wnt Signaling in Osteoblastic Cell Behavior
Author: Juanli Guo
Publisher:ProQuest - 2008

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